Template Switch Oligo

Template Switch Oligo - Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. However, if a large fraction of the library contains a portion. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. The resulting cdna can be amplified by pcr or serve as. However, if a large fraction of the library contains the. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2.

In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. However, if a large fraction of the library contains the. We recommend a tso with rgrgrg at the 3′ end. However, if a large fraction of the library contains a portion. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end.

Template Switch Oligo

A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction of the library contains the. The resulting cdna can be amplified by pcr or serve as. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at.

Template switch and twin priming. (A) Steps describing template

The resulting cdna can be amplified by pcr or serve as. However, if a large fraction of the library contains the. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. We recommend a tso with rgrgrg at the 3′ end. However, if a large fraction of the.

NanoCAGE A HighResolution Technique to Discover and Interrogate Cell

Use this set of oligonucleotides for template switching cdna synthesis and preamplification. However, if a large fraction of the library contains a portion. We recommend a tso with rgrgrg at the 3′ end. The resulting cdna can be amplified by pcr or serve as. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end.

Fig. S12 Schematic representation of the tested oligodT / TSO

In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. A small fraction of single cell 3' libraries are expected to contain the template switching oligo.

(A) The TS mechanism is used for first strand cDNA synthesis. First, an

The resulting cdna can be amplified by pcr or serve as. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction.

(A) First strand cDNA is initiated by priming with an oligo dT primer

Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. However, if a large fraction of the library contains a portion. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. A small fraction of single cell 3' libraries are expected to contain the.

Template Switch Oligo

However, if a large fraction of the library contains the. The resulting cdna can be amplified by pcr or serve as. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. However, if a large fraction of the library contains a portion. We recommend a tso with rgrgrg at the.

Template Switching Oligo

In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. However, if a large fraction of the library contains the. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. The resulting cdna can be amplified by pcr or serve as. A small fraction of visium libraries are.

Tuning 5’ to internal read proportions and template switching oligo PCR

In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. We recommend a tso with rgrgrg at the 3′ end. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at.

Template switching oligos (TS oligos, TSOs) for cDNA library

The resulting cdna can be amplified by pcr or serve as. However, if a large fraction of the library contains a portion. We recommend a tso with rgrgrg at the 3′ end. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. However, if a large fraction of the library contains the.

Template Switching Oligonucleotide And Capturing Oligo (Dt) Are Used To Incorporate Adaptor.

A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction of the library contains the. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers.

The Resulting Cdna Can Be Amplified By Pcr Or Serve As.

(step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. We recommend a tso with rgrgrg at the 3′ end. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end.